licC [2018-06-22 18:17:46]

lichenan-specific phosphotransferase system, EIIC component of the PTS

locus: BSU38580

pI: 8.58

mw: 48.37 kDa

protein length: 452 aa

gene length: 1356 bp

function: lichenan uptake and phosphorylation

product: lichenan-specific phosphotransferase system, EIIC component

essential: no

synonyms: celB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,960,192 → 3,961,550

The protein

Protein family

• [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, lactose permease (Lac) family [Pubmed|10627040]

Paralogous protein(s)

• [protein|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|YwbA], [protein|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|GmuC]

Structure

• [PDB|3QNQ] (EIIC component of diacetylchitobiose specific PTS from ''Bacillus cereus''; 60% identity, 87% similarity) [Pubmed|21471968]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]-[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]-[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]-[gene|84D8AA0AFBF46273F6B4198B49440D55470FEC0F|licH]
  description: [Pubmed|8990303]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8990303], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8990303], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR]: activation, [Pubmed|8990303], in [regulon|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR regulon]

  regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8990303]

Biological materials

Mutant

• BKE38580 (Δ[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATAGCCCGCCCCTCTCT, downstream forward: _UP4_CTGTAGAGAAAGGAAGTGAA
• BKK38580 (Δ[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATAGCCCGCCCCTCTCT, downstream forward: _UP4_CTGTAGAGAAAGGAAGTGAA

References

• 19087206,8990303,16872404,10438772,10627040,8990303